Spider-Man S03E08 - Chapter VIII - The Ultimate Slayer

Angry over his son's arrest and conviction, as well as Spider-Man now knowing his secret identity, to which he blames Alistair Smythe, Kingpin attempts to kill Spider-Man by turning Smythe into a cyborg to let him do it. Smythe's father is revealed to have survived the explosion in this episode, but the Kingpin has put him in cryogenic suspension.